AbstractsBiology & Animal Science

A Transcriptomic Examination of Sexual Differentiation in Zebrafish, Danio rerio

by Stephanie Ling Jie Lee

Institution: University of Otago
Year: 0
Keywords: Zebrafish; Sex differentiation; RNA-Seq; Gonad transcriptome; Brain transcriptome; Sex dimorphic gene expression
Record ID: 1304397
Full text PDF: http://hdl.handle.net/10523/5667


Zebrafish sex determination and sexual differentiation is complex. Neither sex chromosomes, major sex-determining genes nor universal sex-linked markers have been identified, and a variety of environmental factors can influence sex ratios. It is thus unsurprising that the molecular pathways underpinning zebrafish sexual development remain poorly understood. A few key genes such as, SRY-box containing gene 9a (sox9a), anti-Müllerian hormone (amh), cytochrome P450, family 19, subfamily A, polypeptide 1a (cyp19a1a) are known to play roles in zebrafish gonadal differentiation. However, at this time, there are many more questions than answers about sex determination and differentiation in this ubiquitious, and immensely well-studied, species. As with many other vertebrates, sex hormone treatment can induce sex reversal of gonadal phenotype and sexual behaviour in zebrafish. In this study, we take advantage of hormonal manipulation of sex in developing zebrafish and RNA-Seq to unravel the unknown genetic pathways that underlie sex determination and sexual differentiation. We conducted global transcriptomic profiling of juvenile zebrafish brains and gonads at two important developmental stages in gonadal differentiation: (1) juvenile ovary-to-testis transformation (40 days post fertilization) and (2) the completion of testicular and ovarian differentiation (60 days post fertilization). Gene expression profiles from 17α-methyltestosterone masculinised juvenile zebrafish were also compared with untreated zebrafish to improve understanding of androgenic effects on zebrafish sexual differentiation pathways. To our knowledge, this is the first study profiling transcriptome-wide sex dimorphic gene expression in teleost brains during gonadal differentiation. We identified modest sex differences in developing zebrafish brains; 33 and 185 sex dimorphic genes were identified in the brains of 40 dpf and 60 dpf zebrafish respectively. Methyltestosterone treatment significantly altered expression patterns at both timepoints. We observed a total of 1,554 genes differentially expressed between control female brains and MT-treated brains at 40 dpf. 1,379 genes were differentially expressed between 40 dpf male brains and 40 dpf MT-treated brains. At 60 dpf, 728 genes exhibited differential expression between control female brains and MT-treated brains. Interestingly, significantly fewer genes (269) were differentially expressed between control male brains and MT-treated brains at 60 dpf. In contrast, we observed extensive sex differences in zebrafish gonadal transcriptomes. A total of 5,039 genes were sexually dimorphic at 40 dpf. 2,502 and 2,537 genes were up-regulated in transforming testes and juvenile ovaries respectively. At 60 dpf, we identified 4,190 testis-biased genes and 4,267 ovary-biased genes. Many of the sex dimorphic genes identified have no previous links with gonadal differentiation. Despite accelerated testicular development in MT-treated zebrafish, the numbers of differentially expressed genes identified when…