|Institution:||University of Washington|
|Full text PDF:||http://hdl.handle.net/1773/40840|
Developing biologically relevant models of human tissues and organs is an important enabling step for many applications within biological research and medicine. A specific application that physiologically relevant tissue models can be implemented in is drug discovery. In this study, we represent a platform and methodology of generating 3D humanized physiologically relevant skeletal muscle tissues that recapitulate aspects of the native cellular microenvironment found in the native skeletal muscles for the development of a reproducible and high-throughput drug-screening model. This is achieved by utilizing a 3D bioprinting platform in conjugation with human myoblasts-laden decellularized extracellular matrix (dECM) bioinks to form skeletal muscle tissue constructs. Structures that feature a skeletal muscle tissue that is anchored on both sides by rigid structures are printed. The rigid anchor structures would induce passive tension along the skeletal muscle tissue, which influences cellular alignment and orientation along the anchors axis of tension. The results described in this study demonstrate our ability to generate human 3D skeletal muscle tissues in a rapid, high-throughput, and reproducible manner, which can be implemented as a predictive drug screening tool for determining the effects that a novel drug may have in the human body.Advisors/Committee Members: Kim, Deok-Ho (advisor).