AbstractsBiology & Animal Science

Distribution and Characterization of the Soybean Cyst Nematode, Heterodera glycines (HG) Types in South Dakota

by Krishna Acharya

Institution: South Dakota State University
Year: 2016
Keywords: Agricultural Science; Agronomy and Crop Sciences; Plant Pathology
Posted: 02/05/2017
Record ID: 2135076
Full text PDF: http://openprairie.sdstate.edu/etd/967


The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is an endoparasitic nematode and one of the major pests of soybean (Glycine max L.) in the United State and all over the world where soybean is grown. SCN is ranked first among the biological factors that cause yield loss in soybean. The estimated yield loss by this nematode is $1b annually in the United States alone. SCN is thought to have been first found in China. It was first identified in the United States in 1954 in North Carolina and in South Dakota in 1995. To date, SCN continues to spread in South Dakota having been detected in 29 counties. SCN is spread through soil movement when the soil is infested with SCN. The females (cyst) and also eggs remaining outside the cyst are the dispersal units of SCN. The second stage juveniles (J2) (worm-like, infecting stage) hatch from the eggs after stimulation from soybean roots, and infects the soybean roots. SCN not only attacks soybean, but also invades several other leguminous crops such as common bean (Phaseolus vulgaris L.), black bean (Phaseolus vulgaris L.), kidney bean (Phaseolus vulgaris L.), pinto (Phaseolus vulgaris L.) and navy (Phaseolus vulgaris L.) and forage legumes such as vetch (Vicia sativa), lespedeza (Kummerowia sp.), and lupine (Lupinus perennis). Several winter weeds like common chickweed (Stellaria media), henbit (Lamium amplexicaule), purple deadnettle (Lamium purpureum), small-flowered bitter cress (Cardamine hirsuta), shepherd’s-purse (Capsella bursa-pastoris), and field pennycress (Thlaspi arvense) are also hosts for SCN. The losses to soybean production caused by SCN vary mainly with the degree of infestation (population density), susceptibility of the cultivar grown, and other biotic and abiotic factors such as soil type and weather conditions. SCN can cause yield loss of up to 30 % without showing any visible symptoms in the plants and losses can go up to 75% in heavily infested fields. SCN completes its life cycle within 3 to 4 weeks depending upon the environmental factors mainly soil temperature. SCN population genetic diversity is the most challenging aspect for the management of this nematode. This study examined the distribution of Heterodera glycines and characterized HG types prevalent in South Dakota by monitoring the present status, population density, and determining HG types. This study also assessed the resistance of few of the available commercial soybean cultivars against prevalent HG types occur in South Dakota. A total of 250 soil samples were arbitrarily collected from different counties of South Dakota that had previously been found positive for SCN. Analysis of the soil samples showed a high prevalence of SCN infestation (32%) of the soil samples collected for this study and SCN population density varied from 250 to 62,500 per 100 cm3 of soil. The extracted SCN populations were subjected to HG type determination under greenhouse conditions. A total of eight types of Heterodera glycines population were found in South Dakota. Among the eight HG types, HG… Advisors/Committee Members: Emmanuel Byamukama.