AbstractsBiology & Animal Science

Effect of salicylic acid on the expression of heat-shockproteins and morphology of potato (Solanum tuberosum L.) plantsunder heat stress in vitro

by Masoud M Hfidan




Institution: Univerzitet u Beogradu
Department:
Year: 2016
Keywords: Solanum tuberosum L.; salicilna kiselina; toplotnistres; heat-shock proteini; HSP; Cu/ZnSOD
Posted: 02/05/2017
Record ID: 2131353
Full text PDF: https://fedorabg.bg.ac.rs/fedora/get/o:10795/bdef:Content/get


Abstract

Biology - Plant Physiology / Biologija - Fiziologija biljaka The potato, Solanum tuberosum L., is the fourth most important crop in the world. Most of the commercial potato cultivars were developed for growing in temperate climate regions and they are highly sensitive to elevated temperatures. As a result of global warming over next 60 years, significant potato yield losses in the range of 18 to 32% are predicted. These losses can be reduced with adaptations in production methods, such as terms of planting time and use of heat tolerant cultivars. Besides, exploration of compounds that may enhance potato heat tolerance, such as salicylic acid (SA), is an alternative approach to cope with adverse effects of high temperatures on potato growth and yield. The major aim of this study was to investigate effects of SA on the basal heat tolerance in Solanum tuberosum L. Potato heat tolerance correlates with level of small heat-shock proteins (sHSP) accumulation, therefore SA effect on this group of HSPs, as well as HSP101 and Cu/ZnSOD, was investigated. Besides, it was interesting to investigate SA effects on morphological parameters related to plant growth and development. Experiments were conducted in vitro since this experimental setup allowed investigation under strictly controlled conditions, with variation of only selected factors – temperature and SA concentration. Experimental procedure based on potato single-node stem cuttings (SNC) exposure to standard growth temperature (23 °C), excessively elevated temperature (45 °C, 6 h; short-term HS) or prolonged exposure to moderately elevated temperature (35 °C, 20 days; long-term HS) has been established. Similar percentage of plants developed from SNC at 23 °C and during recovery period after exposure to short-term HS, while long-term HS substantially reduced the number of plantlets developed from SNC implying that in potato prolonged, moderately elevated temperatures can cause more devastating effects then excessively elevated ones during short exposure. SA in a range of concentrations from 10-6 M to 10-4 M stimulated development of plants from SNC under long-term HS in all investigated potato cultivars, with the exception of cultivar ‘Marabel’ which did not respond on SA treatment. These findings indicate that SA may enhance basal thermotolerance in potato cultivars/genotypes, as well as variation in genotypes´ sensitivity to SA. SA pretreatments, in the range of concentrations from 10-6 М to 10-4 М, did not affect internode initiation and elongation either under standard or elevated temperatures, while SA treatments in the same range of the concentrations stimulated these processes under both short-term and long-term HS... Advisors/Committee Members: Momčilović, Ivana.