AbstractsChemistry

Gel Electrophoresis, a widely used technique for the separation of proteins in a mixture, must be approached with strict adherence to proper technique for achievement of reliable results. The purpose of the research is to measure the effectiveness of the use of a three-part video as a pre lab preparatory tool to demonstrate to students proper technique prior to completion of the required lab experiment. The video included the processes and steps involved in making the gel, loading the gel, running the gel, and removing and staining the gel. Each semester only half of the class was given access to the video via Blackboard and had two weeks to view it with 24 hour access, while the entire class received the normal pre lab discussion given by graduate TA lab instructor. Both groups were given a questionnaire to be filled out as they completed the lab about the time required to complete each step and their level of confidence on a scale of 1 to 5 in their ability as they completed the steps and their overall confidence in their ability perform the lab while ensuring good or effective results. Prelab questionnaires, Lab questionnaires, instructor evaluations from completed gels, and data retrieved from Blackboard regarding if/when/how many times students actually viewed the video, were analyzed in an attempt to measure the effectiveness of the video as a useful teaching tool.