AbstractsBiology & Animal Science

Quantitative evaluation of characterized ??-L-rhamnosidase effects in sea urchin gastrulation

by Jing Liang

Institution: California State University – Northridge
Department: Department of Biology
Degree: MS
Year: 2015
Keywords: rhamnosidase; Dissertations, Academic  – CSUN  – Biology.
Record ID: 2060592
Full text PDF: http://hdl.handle.net/10211.3/132977


Gastrulation is a fundamental process that mediates formation of ectoderm, mesoderm and endoderm in most animals. Revealing the mechanism of gastrulation will provide a better understanding of embryonic development. Due to its transparency, simplicity of the structure and similarities to complex organisms, the NIH-designated sea urchin embryo model was used in this study. By treating sea urchin embryos with characterized ??-L-rhamnosidase and observation of archenteron development, I investigated the role of L-rhamnose terminal in the mechanism of sea urchin gastrulation. I showed that the ??-L-rhamnosidase treatments used did not contain any detectable contamination and using quantitative protein assay I demonstrated that this enzyme inhibited archenteron development during gastrulation. Denatured and sugar-inhibited ??-L-rhamnosidase lost most of its inhibitory activity. The results combined with a previous study using L-rhamnose, provide convincing evidence, I believe for the first time, for a role of L-rhamnose in the gastrulation process.