AbstractsBiology & Animal Science

The main goal of the study is to produce high levels of the YqgF protein for use in protein-protein interaction studies with YqgE. The specific experimental goals are: 1. To use the Polymerase Chain Reaction (PCR) technique to isolate and amplify the full DNA sequence of yqgF. 2. To clone yqgF into a plasmid that includes a 6X His tag that will attach to the N-terminus of the corresponding YqgF protein. Clones will be tested for the correct orientation and size using Colony PCR and DNA sequencing. 3. To induce high level protein expression of the cloned yqgF genes in E. coli cells. Western Blotting will be used to detect the expressed protein in a crude extract. 4. To purify the YqgF fusion protein.