AbstractsBiology & Animal Science

The Application of Metabolomics to the Evaluation of the Celllular Toxicity

by Yu Wang

Institution: University of Akron
Department: Chemistry
Degree: MS
Year: 2014
Keywords: Biochemistry; Chemistry; metabolomics; liquid chromatography; mass spectrometry; cell toxicity; nanomaterials
Record ID: 2031724
Full text PDF: http://rave.ohiolink.edu/etdc/view?acc_num=akron1397057769


Metabolomics is an emerging field that measures all small molecule species within the cell and their changes in response to genetic reprogramming, environmental toxin exposure, and disease. Currently, the assessment of cell toxicity induced by exposure to environmental contaminants as well as nanomaterials is mainly limited to the measurement of overt cell death in tissue culture or animals. The rapid profiling of multiple biochemical species by liquid chromatography/mass spectrometry (LC/MS) based metabolomics allows the elucidation of early molecular toxicity with one technological platform. In this thesis, we assess the cell toxicity of nanomaterials, polystyrene and L-tyrosine polyphosphate (LTP) as well as two common indoor environmental contaminants, sodium hypochlorite and benzalkonium chloride vapor in the macrophage cell line, THP-1. Utilizing a number of bioinformatics programs including XCMS online, it was found that cells exposed to nanomaterials show upregulation of the lysophosphocholine, 1-heptadecanoyl-sn-glycero-3-phosphocholine which may function as a putative marker of increased fatty acid uptake.