AbstractsBiology & Animal Science

The Role of Retromer in Regulating the Apical-Basal Polarity and the Immune Response during Drosophila Development

by Bo Zhou

Institution: University of Cincinnati
Department: Medicine: Developmental Biology
Degree: PhD
Year: 2012
Keywords: Biology; Retromer; apical-basal polarity; Crumbs; Toll pathway; vps35; Drosophila
Record ID: 1946558
Full text PDF: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1331296365


The retromer complex is a membrane anchored intracellular protein complex.Retromer recognizes specific transmembrane cargos and mediates their transportationfrom endosomes to trans-Golgi network (TGN). Vacuolar protein sorting-associatedprotein 35 (Vps35) is a major component of retromer. Through analyzing phenotypes ofDrosophila vps35 (dvps35) mutants/RNAi, our lab previously identified a role ofretromer in mediating WNT secretion. However, dvps35 mutants/RNAi displayedadditional developmental defects which were not relevant to WNT secretion. Thisindicated additional roles of retromer during Drosophila development. My workpresented here uncovered two additional roles of retromer in (1) regulating apical-basalpolarity in epithelial cells; (2) suppressing auto-activation of the Drosophila immunesystem. Further analysis showed that retromer regulated apical-basal polarity throughrecycling Crumbs, which acted as a novel retromer cargo. I also found that retromersuppressed auto-activation of the immune system by regulating Spatzle, the ligand in theToll signaling pathway. In summary, this dissertation work expanded our understandingof the transmembrane cargos and the biological functions of retromer.