Staphylococcal alpha haemolysin fractions and a study of their immunological and biological properties.
Institution: | McGill University |
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Department: | Department of Biology. |
Degree: | MS. |
Year: | 1959 |
Keywords: | Bacteriology and Immunology. |
Record ID: | 1543740 |
Full text PDF: | http://digitool.library.mcgill.ca/thesisfile112091.pdf |
The purpose of this investigation was to study the biological and immunological properties of purified preparations of Staphylococcus pyogenes Wood 46 alpha toxin. The toxin was fractionated by carboxymethyl cellulose column chromatography and purified by a zinc-ethanol precipitation technique and the materials tested for functional antigenicity in rabbits. An attempt was made to contribute to the question of unity and diversity of biological activities associated with the alpha haemolysin of Staphylococcus pyogenes using the technique of zone electrophoresis on starch-gel.