AbstractsBiology & Animal Science

Milk is a unique food comprising of proteins, carbohydrates, fats, vitamins and newlineminerals. The average pH of the fermented milk by Lactobacillus acidophilus was 5.3 ± newline0.091, commercial curd Aavin was 5.3 ± 0.185, commercial curd Dodla was 5.3 ± 0.126 newlineand Lactobacillus bulgaricus 5.16 ± 0.095. The pH of the control sample, i.e. nonfermented newlinemilk was found to be 7.05. The mean titratable acidity of the fermented milk newlineby commercial curd Dodla was 91.49º T (Toerner s degree), commercial curd Aavin was newline91.27º T, Lactobacillus acidophilus was 92.11º T and Lactobacillus bulgaricus was newline93.52º T. The titratable acidity of the control sample, i.e. non-fermented milk was 50.78º newlineT. The mean viscosity of the milk fermented by commercial curd Dodla was 7.72 mPas newline(Milli Pascal), commercial curd Aavin was 8.01 mPas, Lactobacillus acidophilus was newline7.12 mPas and Lactobacillus bulgaricus was 7.13 mPas. SEM analysis of fermented milk newlinewas carried out to confirm the presence of Lactobacillus species in the fermented milk. newlineAfter the fermentation process was complete in the milk, Isolation of CPP (Casein newlinePhospho Peptides) was done from fermented milk based on enzymatic hydrolysis newlinemethod. Antimicrobial activity of CPP was tested against two common clinical pathogens newlineEscherichia coli (MTCC Number 443) and Pseudomonas sp (MTCC Number 1194) newlineusing zone of inhibition method. AAVIN CPP produced 14 and 16 mm of zone of newlineinhibition with Escherichia coli and Pseudomonas species respectively whereas DODLA newlineCPP produced 13 and 15 mm, L. acido. CPP produced 16 and 15 mm, L. bulg. CPP newlineproduced 12 and 15 mm zone of inhibition with Escherichia coli and Pseudomonas species respectively. HPLC and FTIR Analysis of four CPPs isolated from fermented newlinemilk by two bacterial cultures and two commercial curd inoculums was performed using newlinemilk as the control and they gave characteristic peaks for CPP newline%%%