AbstractsBiology & Animal Science

In order to select improved genotypes with potential for producing high-quality wines in a climate change scenario, asegregating F1 population with 151 progeny derived from a cross between Graciano x Tempranillo was studied for14 agronomic traits, 11 enological traits and 5 seed traits for three consecutive years. All traits presented transgressivesegregation and continuous variation. Significant correlations among traits were observed but most associations were weak.Seven groups of hybrids were distinguished based on ripening time, cluster weight, berry weight and anthocyanins content by cluster analysis; and fourteen genotypes were pre-selected for further research. In addition, the anthocyanin profilesof the hybrids and parents were determined during 2 growing seasons (2009 and2010)with HPLC-MS. Fifteen monoglucosideanthocyanins were detected with HPLC-MS, including two unidentified compounds. The concentration of 13 identified anthocyanins and the percentage of non acylated, acetyl and coumarylanthocyanins were analyzed to understand the inheritance of the anthocyanin profile in the population.Ratios between different anthocyanins were evaluated for their use as potential varietal markers. A genetic linkage map was constructed using Joinmap 3.0 software, following a pseudo-testcross strategy. Altogether271 simple sequence repeat (SSR) and 18071 SNP (Single Nucleotide Polymorphism) markers, and one CAPS marker were screened. Finally, a consensus map with a total of 1210 markers (183SSRs, 1 CAPs and 1026 SNPs) was assembled covering 1385.8 cM distributed into 19 linkage groups, with an average interval length of 1.2cM between markers. Finally a QTL (Quantitative Traits Loci) analysis was carried out using MapQTL 6.0 software with the simple interval mapping (SIM) combined with permutations test and the non-parametric Kruskal-Wallis (KW) for agronomic, enological and seed traits. Con el objetivo de seleccionar nuevos genotipos con el potencial de producir vinos de alta calidad en un escenario de cambio climático, se estudio una población segregante de 151 individuos obtenida del cruzamiento entre Graciano y Tempranillo para 14 caracteres agronómicos, 11 enológicos y cinco caracteres de semilla durante tres años consecutivos. Todos los caracteres presentaron segregación transgresiva y variación continua. Se observaron correlaciones significativas entre los parámetros estudiados pero la mayoría de las asociaciones fueron débiles. Se diferenciaron siete grupos de híbridos basados en fecha de madurez, peso del racimo, peso de la baya y contenido en antocianos, y se seleccionaron catorce genotipos para futuras investigaciones. Además se determinaron los perfiles antociánicos de los híbridos y los parentales durante 2 ciclos de cultivo (2009 y 2010) con HPLC-MS. Se detectaron 15 compuestos con HPLC-MS, incluyendo dos compuestos no identificados. La concentración de 13 antocianos identificados y el porcentaje de antocianos no acilados, acetilados y cumarilados fueron analizados para comprender la herencia del perfil…