AbstractsBiology & Animal Science

Application of proteomics and cytomics in human neutrophils functional studies

by Alexandre Rosa Campos

Institution: Universidade de Brasília
Year: 2007
Keywords: PROTEINAS; neutrófilos; proteômica subcelular; citometria de fluxo; stress oxidativo; citômica; proteômica
Record ID: 1077778
Full text PDF: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2832


Biomedical research commonly starts by raising a hypothesis to solve a problem. In this context, scientists select the most appropriate method(s) to answer the question and solve a common dilemma. Over the past decade, we have witnessed a revolution of new technologies in molecular biology the Omics Science. Highscale technologies such as metabolomics, cytomics, genomics and proteomics are changing the way we study complex biological systems. Current approaches to understanding the functional diversity of an organism preferentially strive for a systems biology approach whereby first the phenotypic classification of a specific cytome is achieved prior to an attempt to perform proteomic analysis. In this context, to better understand the features that involve neutrophil activation and programmed cell death in the pathological and healthy states, this study proposes the integration of cell biology approaches such as flow cytometry with a very robust proteomics platform in an attempt to integrate data at the molecular level with phenotypic data of neutrophils. The application of subcellular fractionation method using digitonin detergent extraction to enrich cytosolic proteins from neutrophils was found reproducible, simple to perform, and inexpensive.